非编码小RNA （RyhB）调控禽致病性大肠杆菌毒力相关基因的分析

doi:10.11843/j.issn.0366-6964.2015.08.017

Abstract

Abstract:

Taking small RNA（RyhB）as the object of study，the experiment explored the regulation of RyhB on avian pathogenic Escherichia coli (APEC) virulence-related genes.The Red homologous recombination method was used to construct the RyhB gene deletion strain (△RyhB) and the complemented strain (△RyhB-comp) by transforming the fusion plasmid pSTV28-RyhB into △RyhB.Viable bacteria counting method was used to evaluate the effects of AE17，△RyhB and △RyhB-comp on the capability of APEC to adhere and invade DF-1 cell.Their effects on the mRNA levels of the eight virulence genes were analyzed by Real-time PCR.The results showed that APEC strains △RyhB and △RyhB-comp were constructed successfully.The adherence of △RyhB was decreased by 62.5% compared to AE17，and that of △RyhB-comp was increased about 1.4 folds compared to △RyhB.Real-time PCR revealed that the transcription of bcfA, fyuA，tsh，luxS，fimC，ompA genes of △RyhB were decreased by 19%，52%，20%，14%，28% and 52%，respectively；while the iss and ibeA genes were increased about 1.14 folds and 1.2 folds respectively.It showed that the deletion of RyhB can reduce the adhesive power of APEC to DF-1 cells and decrease the transcription of virulence genes.Our study demonstrates that RyhB plays a very important role in regulating the virulence of APEC.

CLC Number:

S852.612

YIN Lei，QI Ke-zong，TU Jian，ZHOU Xiu-hong，LIU Hong-mei，WANG Man，ZHANG Yan-na. Analysis of the Regulation of Small Non-coding RNA (RyhB) on Avian Pathogenic Escherichia coli Virulence-related Genes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1409-1416.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.08.017

https://www.xmsyxb.com/EN/Y2015/V46/I8/1409

References

［1］SCHOULER C，SCHAEFFER B，BRÉE A，et al.Diagnostic strategy for identifying avian pathogenic Escherichia coli based on four patterns of virulence genes［J］.J Clin Microbiol ，2012，50(5):1673-1678.
［2］WASSARMAN K M，ZHANG A，STORZ G.Small RNAs in Escherichia coli［J］.Trends Microbiol，1999，7(1)：37-45.
［3］WATERS L S，STORZ G.Regulatory RNAs in bacteria［J］.Cell，2009，136(4)：615-628.
［4］MASSÉ E,VANDERPOOL C K，GOTTESMAN S.Effect of RyhB small RNA on global iron use in Escherichia coli［J］.J Bacteriol，2005，187(20):6962-6971.
［5］VECEREK B，MOLL I，BLÄSI U.Control of Fur synthesis by the non-coding RNA RyhB and iron-responsive decoding［J］.EMBO J，2007，26(4):965-975.
［6］MASSÉ E，GOTTESMAN S.A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli［J］.Proc Natl Acad Sci USA，2002，99(7) ：4620-4625.
［7］WASSARMAN K M，REPOILA F，ROSENOW C，et al.Identification of novel small RNAs using comparative genomics and microarrays［ J ］.Genes Dev，2001，15(13):1637-1651.
［8］MEY A R，CRAIG S A，PAYNE S M.Characterization of Vibrio cholerae RyhB：the RyhB regulon and role of ryhB in biofilm formation［J］.Infect Immun，2005，73(9):5706-5719.
［9］DAVIS B M，QUINONES M，PRATT J，et al.Characterization of the small untranslated RNA RyhB and its regulon in Vibrio cholerae［J］.J Bacteriol，2005,187(12):4005-4014.
［10］OGLESBY A G，MURPHY E R，IYER V R，et al.Fur regulates acid resistace in Shigella flexneri via RyhB and ydeP［J］.Mol Microbiol，2005，58(5)：1354-1367.
［11］WILDERMAN P J，SOWA N A，FITZGERALD D J，et al.Identification of tandem duplicate regulatory small RNAs in Pseudomonas aeruginosa involved in iron homeostasis［J］.Proc Natl Acad Sci USA，2004,101(26)：9792- 9797.
［12］HERNÁNDEZ J A，MURO-PASTOR A M，FLORES E，et al.Identification of a furA cis antisense RNA in the cyanobacterium Anabaena sp.PCC 7120［J］.J Mol Biol，2006，355(3)：325-334.
［13］MURPHY E R，PAYNE S M.RyhB，an iron-responsive small RNA molecule，regulates Shigella dysenteriae virulence［J］.Infect Immun，2007，75(7)：3470-3477.
［14］MUYRERS J P，ZHANG Y,STEWART A F.Techniques：Recombinogenic engineering-new options for cloning and manipulating DNA［J］.Trends Biochem Sci，2001，26(5):325-331.
［15］GRIPENLAND J，NETTERLING S，LOH E，et al.RNAs：regulations of bacterial virulence［J］.Nat Rev Microbiol，2010，8(12):857-866.
［16］JONES C H，PINKNER J S，NICHOLES A V，et al.FimC is a periplasmic PapD-like chaperone that directs assembly of type 1 pili in bacteria［J］.Proc Natl Acad Sci USA，1993，90(18):8397-8401.
［17］KINT G，DE C D，MARCHAL K，et al.The small regulatory RNA molecule MicA is involved in Salmonella enterica serovar Typhimurium biofilm formation［J］.BMC Microbiol，2010，10:276.

[1]	ZHI Yan, MEI Chen, LIU Zhenyi, WUYUN Gerile, WANG Hongjun, HU Ge. Research Progress on the Virulence Factors of Avibacterium paragallinarum [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4934-4942.
[2]	WANG Jianing, ZHANG Ziqiang, KONG Dejing, FENG Caicai, ZHANG Feike, LIU Yumei. Isolation and Identification of Klebsiella pneumoniae in Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5198-5206.
[3]	LI Lili, CHEN Kaifeng, CHEN Bing, ZHOU Zhouping, WANG Nanwei, QU Xiaoyun, XU Chenggang, LIAO Ming, ZHANG Jianmin. Regulatory Role of STM1827 in the Biofilm Formation and Environmental Stress of Salmonella Typhimurium [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5207-5217.
[4]	TONG Panpan, HUANG Shunmin, WANG Yudan, SHI Xuhui, CHEN Wenxia, SONG Xinlong, ZHANG Yi, SU Zhanqiang, XIE Jinxin. Phylogenetic Clustering, Serotype and Drug Resistance Analysis of Escherichia coli from Diarrhea with Piglets in Xinjiang [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 414-420.
[5]	WANG Fei, YANG Jie, Lü Qingjie, WANG Mixue, LIU Peng, ZHANG Ruoyu, SHI Congcong, WANG Xueying, LIN Lin, HUA Lin, SONG Wenbo, LIANG Wan, CHEN Huanchun, WU Bin, PENG Zhong. Isolation and Genomic Characterization of a Meningitis Causing Pasteurella multocida [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4346-4355.
[6]	MEN Kaikai, LIU Jialong, GUO Yage, HE Lei, JIA Yanyan, YU Zuhua. Effects of Chicken TGF-β1 on the Adhesion of Escherichia coli and Salmonella pullorum to DF1 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4000-4007.
[7]	YANG Yang, XIE Liqing, HU Pei, GAO Lixu, YUAN Xiang, LI Pan, PENG Yuanyi, LI Nengzhang. Construction of Bovine Pasteurella multocida Type A hyaD Mutant and Its Cross-protection Analysis in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3582-3597.
[8]	SHAO Ying, FU Dandan, WU Xiaoyan, GU Yi, SONG Xiangjun, TU Jian, QI Kezong. Effect of the ETT2 Structural Gene epaPQR on the Biological Properties and Pathogenicity of Avian Pathogenic Escherichia coli [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3631-3641.
[9]	XU Wenbo, WU Limei, LIU Xin, LI Sheng, HUANG Fushen, LI Runcheng. Analysis on Complete Genome Sequence and Pathogenic Genes of a Pasteurella multocida Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1858-1869.
[10]	WANG Xi, LI Ke, LI Tingcui, YAN Hongya, ZHAO Rong, CHANG Zhishun, LIAO Ming, SUN Minhua, XIN Aiguo. MLST Typing and Drug Resistance Analysis of 75 Salmonella Strains Isolated from Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1626-1631.
[11]	WANG Jun, LI Jun, CUI Guolin. The Effect of R91S Mutation in FliC on the Flagellar Shape and Salmonella Enteritidis Colonization in BALB/c Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 607-617.
[12]	TONG Panpan, ZHANG Mengmeng, CHEN Wenxia, LIU Luyao, ZHANG Ling, TANG Xuelin, SU Zhanqiang, XIE Jinxin. Phylogenetic Clustering, Serotype, Virulence Genes and Drug Resistance Analysis of Shiga Toxin-producing Escherichia coli from Cattle, Sheep and Camel in Xinjiang [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3660-3668.
[13]	CHEN Chaoqun, CHEN Jiali, ZHOU Xuanyi, WU Xue, WANG Jincheng, HUANG Anxiong, HAO Haihong. Establishment of Epidemiological Cut-off Values and Determination of Drug Resistance of Haemophilus parasuis with β-lactam Drugs [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3234-3245.
[14]	HE Zhuolin, TANG Minjia, ZHANG Xuejing, HOU Xiao, PU Wanxia. ERIC-PCR Genotyping and Phylogenetic Grouping of Escherichia coli from a Dairy Farm Environment [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 2059-2064.
[15]	YI Zhengfei, ZHANG Yaodong, WANG Yao, ZHU Hong, TAO Chenglin, AFAYIBO Dossêh Jean Apôtre, LI Tao, QI Jingjing, TIAN Mingxing, DING Chan, YU Shengqing, WANG Shaohui. Two Component System CpxR Contributes to the Antibiotic Resistance, Serum Bactericidal Resistance and Virulence of Avian Pathogenic Escherichia coli [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1053-1060.

Analysis of the Regulation of Small Non-coding RNA (RyhB) on Avian Pathogenic Escherichia coli Virulence-related Genes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments